ANTI-PHOTOAGING ACTIVITY OF KOMBUCHA COFFEE (Coffea canephora) EXTRACT ANTIOXIDANT MOISTURIZER ON ULTRAVIOLET-B INDUCED MALONDIALDEHYDE IN MICE SKIN

Authors

• Renata Prameswari, Department of Dermatology and Venereology, Faculty of Medicine, Hang Tuah University, Surabaya, IndonesiaFollow
• Lifia Chandra, Faculty of Medicine, Hang Tuah University, Surabaya, Indonesia
• Dian Ardiana, Department of Dermatology and Venereology, Faculty of Medicine, Hang Tuah University, Surabaya, Indonesia

ORCID ID

Renata Prameswari: https://orcid.org/0009-0008-1103-3066

Dian Ardiana: https://orcid.org/0000-0003-4112-8601

Abstract

Photoaging caused by chronic sun exposure exhibits characteristics similar to intrinsic aging, including skin thinning, moisture loss, roughness, collagen degradation, and wrinkle formation. This process is driven by oxidative stress and the formation of malondialdehyde (MDA) as a result of lipid peroxidation. Topical intervention using antioxidant-based moisturizers could prevent further damage. Robusta coffee (Coffea canephora), high levels of chlorogenic acid and caffeine, is known for its antioxidant properties. When fermented with a Symbiotic Culture of Bacteria and Yeast (SCOBY) to produce kombucha coffee, its bioactive compounds are enhanced, potentially offering additional protection against oxidative stress and photoaging. This research aims to evaluate the preventive role of kombucha coffee extract as an antioxidant moisturizer against ultraviolet B radiation (UV-B) induced malondialdehyde in mice. Thirty male mice were randomly divided into six groups (n=5 each): control (no irradiation or application), negative control (UV-B only), positive control (UV-B + base moisturizer), and three treatment groups receiving UV-B plus kombucha coffee extract moisturizers at 5%, 10%, and 15%. UV-B irradiation at 70 mJ/cm² was administered for 14 consecutive days. Moisturizers were applied topically 20 minutes before and 4 hours after irradiation. Skin MDA levels were then measured and statistically analyzed. The exposure of UV-B significantly increased MDA levels in the negative control group (2522.25 nmol/mg) compared to the non-irradiated control (320.5 nmol/mg). Kombucha coffee extract moisturizer reduced MDA levels in a concentration-dependent manner, with the lowest values observed in the 10% extract group (872 nmol/mg), followed by 5% (914.25 nmol/mg) and 15% (1309.75 nmol/mg). Mean MDA levels in all groups were significant difference (p = 0.001), indicating the reducing malondialdehyde in mice dorsal skin effect. Kombucha coffee extract formulated as an antioxidant moisturizer effectively impede photoaging and alleviate skin MDA in mice.

Keywords

Antioxidant moisturizer; Health innovation; Kombucha coffee; Malondialdehyde; Photoaging; Ultraviolet-B radiation

First Page

56

Last Page

62

DOI

10.65346/2599-056X.2429

Publication Date

6-30-2026

Recommended Citation

Prameswari, Renata; Chandra, Lifia; and Ardiana, Dian. 2026 ANTI-PHOTOAGING ACTIVITY OF KOMBUCHA COFFEE (Coffea canephora) EXTRACT ANTIOXIDANT MOISTURIZER ON ULTRAVIOLET-B INDUCED MALONDIALDEHYDE IN MICE SKIN. Folia Medica Indonesiana. 62, 1 (Jun. 2026 ), 56-62.
Available at: https://doi.org/10.65346/2599-056X.2429