Microbiological Assessment of Fresh Expressed Breast Milk on Room Temperature at Dr. Soetomo Hospital Neonatal Unit

Authors

Nur Aisyah Widjaja, Division of Nutrition and Metabolic Disease, Departement of Pediatric, Faculty of Medicine, Universitas Airlangga, Surabaya
Kartika Hardiyani, Division of Nutrition and Metabolic Disease, Departement of Pediatric, Faculty of Medicine, Universitas Airlangga, Surabaya
Meta Herdiana Hanindita, Division of Nutrition and Metabolic Disease, Departement of Pediatric, Faculty of Medicine, Universitas Airlangga, Surabaya
Roedi Irawan, Division of Nutrition and Metabolic Disease, Departement of Pediatric, Faculty of Medicine, Universitas Airlangga, Surabaya
Eddy Bagus Wasito, Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Hospital, Surabaya
Maria Inge Lusida, Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo Hospital, Institute of Tropical Disease, Universitas Airlangga, Surabaya

Abstract

Storing EBM at room temperature in several hours before consuming, frequently found in Indonesia. Based on Academy of Breastfeeding Medicine guidelines EBM can last for 6 to 8 hours in room temperature (25oC or 77oF). However, currently there hasn't been study in tropical country especially Indonesia for the guidelines. This study aimed to assess microbiological quality of EBM on room temperature, including bacterial growth and major bacterial found on EBM for health care and society recommendations. An observational study of 30 expressed breast milk samples provided by 30 healthy women with term baby below 6 month old. The samples were kept sterile and laid at plates for 0 hours, 2 hours, 4 hours and 6 hours in room temperature (26°-32° C) and used drop plate technique on several culture media. Data was analyzed by Chi-square and paired sample T-test. Thirty of unheated fresh EBM from 30 lactating mothers were stored at room temperature, examined for the degree of bacterial contamination at 0 hour, 2 hours, 4 hours, and 6 hours. All the EBM samples were contaminated at 2 hour. Bacterial species identified was Coagulase-negative Staphylococcus (CNS), Escherichia coli, Klebsiella pneumoniae and Streptococcus faecalis, range of growth 109 cfu/ml-63 x 109 cfu/mm3 after 6 hour of storage. The EBM exposed at room temperature (30-36 0C) for more than two hour reduce the quality and do not recommended to be given to the infants.

First Page

30

Last Page

36

DOI

10.20473/fmi.v55i1.24346

Publication Date

3-31-2019

Recommended Citation

Widjaja, Nur Aisyah; Hardiyani, Kartika; Hanindita, Meta Herdiana; Irawan, Roedi; Wasito, Eddy Bagus; and Lusida, Maria Inge (2019) "Microbiological Assessment of Fresh Expressed Breast Milk on Room Temperature at Dr. Soetomo Hospital Neonatal Unit," Folia Medica Indonesiana: Vol. 55: No. 1, Article 6.
DOI: 10.20473/fmi.v55i1.24346
Available at: https://scholarly.unair.ac.id/fk-fmi/vol55/iss1/6