Effects of Arak Bali Administration on Spermatozoa DNA Fragmentation and Testosterone Level of Rats (Rattus norvegicus)

Authors

Alfiah Hayati, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya
Dwi Winarni, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya
Ni Wayan Sukma Antari, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya
Inne Ninda Resita, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya
Erlyn Nurul Fauziah, Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya
Mentari Octarina Ariadnya, Faculty of Medicine, Universitas Airlangga, Surabaya
Nikita Gladys Larasati, Faculty of Medicine, Universitas Airlangga, Surabaya
Nur Lia F Darain, Faculty of Medicine, Universitas Airlangga, Surabaya
Ovitrani Nanda, Faculty of Medicine, Universitas Airlangga, Surabaya
Silvi Mavita, Faculty of Medicine, Universitas Airlangga, Surabaya
Usamah Usamah, Faculty of Medicine, Universitas Airlangga, Surabaya
Jongky Hendro Prajitno, Endocrine & Metabolism Division, Department of Internal Medicine, Faculty of Medicine & Dr. Soetomo General Hospital, Surabaya

Abstract

This study aimed to determine the effects of arak bali on the fragmentation of spermatozoa and testosterone in rats (Rattus norvegicus). This study used 24 rats (170-200 g), divided into four groups: one control and three treatments (receiving arak bali containing 40% alcohol as much as 0.1 and 0.5 mL and synthetic alcohol (40%) as much as 0.1 mL, for 45 days). The observation of DNA fragmentation was done using acridine orange staining and the measurement of testosterone level used ELISA method. The results showed that the provision of arak bali in experimental animals increased the occurrence of spermatozoa DNA fragmentation. The higher the volume of arak bali given, the higher the fragmentation of spermatozoa DNA. The administration of arak bali also decreased testosterone level. The higher the volume of arak bali given, the lower the rats' testosterone level.

Keywords

Arak bali, spermatozoa DNA fragmentation, testosterone, rats (Rattus norvegicus)

First Page

41

Last Page

45

DOI

10.20473/fmi.v54i1.8051

Publication Date

4-5-2018

Recommended Citation

Hayati, Alfiah; Winarni, Dwi; Antari, Ni Wayan Sukma; Resita, Inne Ninda; Fauziah, Erlyn Nurul; Ariadnya, Mentari Octarina; Larasati, Nikita Gladys; Darain, Nur Lia F; Nanda, Ovitrani; Mavita, Silvi; Usamah, Usamah; and Prajitno, Jongky Hendro (2018) "Effects of Arak Bali Administration on Spermatozoa DNA Fragmentation and Testosterone Level of Rats (Rattus norvegicus)," Folia Medica Indonesiana: Vol. 54: No. 1, Article 8.
DOI: 10.20473/fmi.v54i1.8051
Available at: https://scholarly.unair.ac.id/fk-fmi/vol54/iss1/8